Lamp2 staining
TīmeklisLUNG - Antibody staining i Antibody staining in the annotated cell types in the current human tissue is reported as not detected, low, medium, or high, based on … TīmeklisMalfunctioning LAMP2 (classified as Danon's disease) and adenosine monophosphate (AMP)-activated kinase gamma 2 proteins (PRKAG2) can result in glycogen accumulation in heart and skeletal muscle. Between the ages of 8 and 15 years, patients with LAMP2 deficiency typically present with chest pain, heart palpitations, syncope, …
Lamp2 staining
Did you know?
Tīmeklis2024. gada 3. sept. · Neurons at DIV7 were stained with BODIPY–pepstatin A (1 µM) for 1 h followed by fixation and immunostaining with antibodies against LAMP1 and CI-M6PR. White arrows indicate LAMP1-labeled organelles with active cathepsin D, orange arrows point to nondegradative LAMP1 organelles that are costained by the late … Tīmeklis2024. gada 9. apr. · Lamp2 immunostaining showed consistent results with the RFP–GFP tandem protein assay (Fig. 2g, Supplementary Fig. 6). Note that although etoposide activates both autophagy and apoptosis, we ...
TīmeklisLAMP2 stain: ATPase pH 9.4 stain: Fiber types. Aggregates occur in both type 1 & type 2 muscle fibers NADH stain: Internal architecture. Abnormally punctate Small, irregular, internal clear regions Patient: … Tīmeklis2024. gada 17. aug. · Immunostaining of p27 in digitonin-permeabilized cells, to remove soluble p27, showed significant colocalization of p27 with two lysosomal …
Tīmeklis2024. gada 12. nov. · Lysosome-associated membrane protein type 2A (LAMP2A) is a key protein in the chaperone-mediated autophagy (CMA) pathway. LAMP2A helps in lysosomal uptake of modified and oxidatively damaged proteins directly into the lumen of lysosomes for degradation and protein turnover. TīmeklisStatistical analysis was performed using one-way ANOVA. (E) Representative human LAMP2 immunofluorescence staining of heart sections from WT and Lamp2 KO mice treated with PBS or AAV9.LAMP2B (1 × 10 13, 5 × 10 13, 1 × 10 14, and 2 × 10 14 vg/kg). Dystrophin was used to mark and localize cardiac myocytes.
TīmeklisDue to enhanced expression of LAMP2 glycoproteins caused by LAMP1 gene deficits, LAMP1 and LAMP2 expression are related (11). Therefore, it is believed that the two have comparable in vivo functions.
Tīmeklis(E) C2C12 cells were transfected with pcDNA3.1-SLC38A9 construct or siRNA to overexpress or knockdown SLC38A9 and stained with anti-SLC36A1 or anti-LAMP2. Scale bar: 10µM. (F) Ratio of SLC36A1 to LAMP2 (%). To assess SLC36A1 translocation, the numbers of SLC36A1 and LAMP2-positive spots per cell were … sbga phone numberTīmeklis2024. gada 26. dec. · Tissue was stained for LAMP1, LAMP2, CTSD (cathepsin D), and SQSTM1 (p62). Nuclei were stained with DAPI. (A) The trophoblast layer in NP, not PE, tissue, was significantly stained for LAMP1. Analysis of LAMP1 staining in NP and PE tissue samples suggested statistically significant differences. (B) LAMP2 staining in … should middle schoolers dateTīmeklis2015. gada 10. dec. · The two left panels represent images of marker IHC staining for LAMP2 in two magnifications. ( d) IHC staining images superimposed over the … sbgc hostsTīmeklisStaining pattern of p62, LC3 and LAMP2 in IMNM and sIBM. LC3 (A, B) and p62 (D, E) stains in IMNM skeletal muscle biopsies show a fine granular pattern throughout the entire sarcoplasm with... should military rank be capitalizedTīmeklisCells were stained with monoclonal antibodies directed against two of the major LAMPs, lamp1 and lamp2, and after subsequent staining with a fluorescent second … should midnight be capitalizedTīmeklisSKIN 2 - Antibody staining i Antibody staining in the annotated cell types in the current human tissue is reported as not detected, low, medium, or high, based on … should midwest be capitalized in a sentenceTīmeklis2024. gada 23. marts · For GRP78 and LAMP2 staining, the oocytes after blocking were incubated with rabbit polyclonal anti-LAMP2 antibody and rabbit polyclonal anti-GRP78 (1:100) at 4°C overnight, and then the oocytes were washed by wash buffer (0.1% Tween 20 and 0.01% Triton X-100) three times (2 min each time). Next the … should middle schoolers be saying the n words